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earle’s balanced salt solution ebss #24010043  (Thermo Fisher)


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    Thermo Fisher earle’s balanced salt solution ebss #24010043
    Earle’s Balanced Salt Solution Ebss #24010043, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/earle’s balanced salt solution ebss #24010043/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    earle’s balanced salt solution ebss #24010043 - by Bioz Stars, 2026-06
    90/100 stars

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    ebss #24010043  (Thermo Fisher)
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    earl’s balanced salt solution (ebss) 24010043  (Thermo Fisher)
    90
    Thermo Fisher earl’s balanced salt solution (ebss) 24010043
    Mitochondrial morphology is altered in Nrg4 KD adipocytes. ( a ) Expression of optic atrophy 1 ( Opa1 ) and mitofusin 2 ( Mfn2 ) in scrambled (Scr) and Nrg4 knockdown (KD) adipocytes at day 7 (D7) of differentiation (n = 6). QPCR data were normalized to Scr adipocyte expression at D7. ( b ) Quantification and representative Western blot of MFN2 and OPA1 in Scr and Nrg4 KD adipocytes at D7 (n = 3). Each lane represents an independent experiment. ( c ) Mitochondrial micrographs of Scr and Nrg4 KD adipocytes at D7. Images were taken with a Leica TCS-SPE confocal microscopy (×63). Mitochondria were stained with the MitoTracker TM red probe. ( d ) Quantification of adipocytes at D7 with elongated (>4 μm), fragmented (1–4 μm), and condensed (<1 μm) mitochondrial network. The percentage over a total of 10 adipocytes is represented. ( e ) Quantification and representative blot of MFN2 and TIM44 in non-treated and bafilomycin A1 (Baf)-treated Scr and Nrg4 KD adipocytes at D7 (n = 3). Adipocytes were starved for 2 h with <t>EBSS</t> <t>media</t> without serum. Cells were then treated with 200 nM Baf for 2 h. Each lane represents an independent experiment. Data are mean ± SEM; * p < 0.05, ** p < 0.01, and ns (not significant).
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    Mitochondrial morphology is altered in Nrg4 KD adipocytes. ( a ) Expression of optic atrophy 1 ( Opa1 ) and mitofusin 2 ( Mfn2 ) in scrambled (Scr) and Nrg4 knockdown (KD) adipocytes at day 7 (D7) of differentiation (n = 6). QPCR data were normalized to Scr adipocyte expression at D7. ( b ) Quantification and representative Western blot of MFN2 and OPA1 in Scr and Nrg4 KD adipocytes at D7 (n = 3). Each lane represents an independent experiment. ( c ) Mitochondrial micrographs of Scr and Nrg4 KD adipocytes at D7. Images were taken with a Leica TCS-SPE confocal microscopy (×63). Mitochondria were stained with the MitoTracker TM red probe. ( d ) Quantification of adipocytes at D7 with elongated (>4 μm), fragmented (1–4 μm), and condensed (<1 μm) mitochondrial network. The percentage over a total of 10 adipocytes is represented. ( e ) Quantification and representative blot of MFN2 and TIM44 in non-treated and bafilomycin A1 (Baf)-treated Scr and Nrg4 KD adipocytes at D7 (n = 3). Adipocytes were starved for 2 h with EBSS media without serum. Cells were then treated with 200 nM Baf for 2 h. Each lane represents an independent experiment. Data are mean ± SEM; * p < 0.05, ** p < 0.01, and ns (not significant).

    Journal: International Journal of Molecular Sciences

    Article Title: Neuregulin 4 Downregulation Alters Mitochondrial Morphology and Induces Oxidative Stress in 3T3-L1 Adipocytes

    doi: 10.3390/ijms252111718

    Figure Lengend Snippet: Mitochondrial morphology is altered in Nrg4 KD adipocytes. ( a ) Expression of optic atrophy 1 ( Opa1 ) and mitofusin 2 ( Mfn2 ) in scrambled (Scr) and Nrg4 knockdown (KD) adipocytes at day 7 (D7) of differentiation (n = 6). QPCR data were normalized to Scr adipocyte expression at D7. ( b ) Quantification and representative Western blot of MFN2 and OPA1 in Scr and Nrg4 KD adipocytes at D7 (n = 3). Each lane represents an independent experiment. ( c ) Mitochondrial micrographs of Scr and Nrg4 KD adipocytes at D7. Images were taken with a Leica TCS-SPE confocal microscopy (×63). Mitochondria were stained with the MitoTracker TM red probe. ( d ) Quantification of adipocytes at D7 with elongated (>4 μm), fragmented (1–4 μm), and condensed (<1 μm) mitochondrial network. The percentage over a total of 10 adipocytes is represented. ( e ) Quantification and representative blot of MFN2 and TIM44 in non-treated and bafilomycin A1 (Baf)-treated Scr and Nrg4 KD adipocytes at D7 (n = 3). Adipocytes were starved for 2 h with EBSS media without serum. Cells were then treated with 200 nM Baf for 2 h. Each lane represents an independent experiment. Data are mean ± SEM; * p < 0.05, ** p < 0.01, and ns (not significant).

    Article Snippet: Calf serum (#16170078), fetal bovine serum (FBS) (#10270106), penicillin/streptomycin (#15140122), EBSS media (#24010043), and puromycin (#A1113803) were purchased from Gibco (Tavarnuzze, Italy).

    Techniques: Expressing, Knockdown, Western Blot, Confocal Microscopy, Staining